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    Illumina Stranded Total RNA Prep with Ribo-Zero Plus or Ribo-Zero Plus Microbiome

    Streamlined RNA-Seq solution for clear and comprehensive analysis across the coding and non-coding transcriptome with exceptional study flexibility.

    ~7 hr

    Assay time

    < 3 hr

    Hands-on time

    1–1000 ng…

    Input quantity

    See full details in the specifications table

    Overview

    Illumina Total RNA Prep with Ribo-Zero Plus provides accurate measurement of gene and transcript abundance and detects both known and novel features in coding and multiple forms of noncoding RNA. Researchers can have confidence in discovery of alternative transcripts, gene fusions, and allele-specific expression. Additionally, the Illumina Stranded Total RNA Prep with Ribo-Zero Plus Microbiome delivers high-quality data for metatranscriptomics applications.

    Key features

    • Exceptional performance—Removes prokaryotic and eukaryotic ribosomal RNA (rRNA) and globin RNA from multiple species in a single reaction to allow rich transcriptome analysis

    • High-quality data—Achieves high-sensitivity from low RNA inputs (1–1000 ng) and difficult sample types, including formalin-fixed paraffin-embedded (FFPE), stool samples, and other low-quality samples

    • Fast, flexible workflow—Prepares libraries 40% faster than comparable kits. Innovations to the workflow, including shorter incubation times and reduced sample cleanup steps, result in a total assay time of 7 hr

    Specifications

    Assay time ~7 hr
    Automation capability Liquid handling robots
    Content specifications Captures coding RNA plus multiple forms of non-coding RNA
    Description For the preparation of whole-transcriptome sequencing-ready libraries from low RNA inputs and low-quality samples that supports a wide range of RNA-Seq applications. Includes Ribo-Zero Plus for single-tube depletion of rRNA and globin RNA from multiple species to facilitate rich transcriptome analyses. 
    Hands-on time < 3 hr
    Input quantity 1–1000 ng total RNA for standard-quality RNA samples. 10 ng total RNA minimum recommended for optimal performance and FFPE samples 
    Instruments NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, NovaSeq X System, NextSeq 500 System, NovaSeq 6000 System, NovaSeq X Plus System
    Mechanism of action Enzymatic rRNA depletion, ligation-based addition of adapters and indexes
    Method Whole-transcriptome sequencing, Metatranscriptome sequencing
    Multiplexing Up to 384 Unique Dual Indexes (UDIs)
    Nucleic acid type RNA
    Specialized sample types Microbiome samples, Low-input samples, FFPE tissue
    Species category Mouse, Human, Rat, Bacteria
    Species details Ribo-Zero Plus depletes abundant transcripts from multiple species including: human cytoplasmic and mitochondrial rRNA, mouse rRNA, rat rRNA, bacteria (E. coli and B. subtilis) rRNA, and human beta globin transcripts  Ribo-Zero Plus Microbiome depletes abundant transcripts from multiple species including: those depleted in Ribo-Zero Plus as well as diverse microbial species and ATCC MSA-2002, MSA-2005, and MSA-2006 
    Strand specificity Stranded
    Technology Sequencing
    Variant class Single nucleotide polymorphisms (SNPs), Gene fusions, Novel transcripts, Transcript variants

    Required products

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    FAQs

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